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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
15/09/2009 |
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Data da última atualização: |
24/02/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, C. F.; DRIESSEN, K.; COSTA, P. M.; CARVALHO-NETO, J. O.; SOUSA, R. V.; RUMPF, R.; DODE, M. N. |
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Afiliação: |
Carlos Frederico Martins, CPAC; Katlen Driessen, UnB; P. Melo Costa; J.O. Carvalho-Neto Cenargen; R.V. de Sousa, Cenargen; R. Rumpf, Cenargen; M.N. Dode, Cenargen. |
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Título: |
Recovery, cryopreservation and fertilization potential of bovine spermatozoa obtained from epididymides stored at 5 .C by different periods of time |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 116, n. 1-2, nov. 2009, p. 50-57 |
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Idioma: |
Inglês |
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Conteúdo: |
The objetive of the present study was to evaluate the effect of the interval between animal' s death and sperm recovery on the freezability and fertilizing ability of spermatozoa frombull epididymides stored for different periods of time. Testis from 25 bulls were collected at the abattoir 2 h after the slaughter. In the laboratory spermatozoa from one epididymis were recovered and analysed for motility. The remaining epididymis was stored for 24 h (G24), 48 h (G48) and 72 h (G72) at 5 .C. At the end of each time period, spermatozoa were recuperated and cryopreserved in Tris-egg yolk and glycerol. Pre-freeze and post-thaw sperm samples were taken to assess total and progressive motility, concentration, membrane integrity and acrosome integrity. For evaluation of fertilizing ability, in each time period five straws of each bullwere thawed, pooled
and used for in vitro embryo production. The results showed that after 48 h of storage there was a decline in total motility, which did not change until 72 h. Progressive motility, plasma membrane and acrosome integrity were not affected by any of the storage periods.
Conversely, all sperm parameters, except progressive motility, were reduced after cryopreservation. Embryo production was less (P < 0.05) in the treatments than in the reference group. However, there was no differences (P > 0.05) in blastoycst rate among
experimental groups. Considering all the embryos produced by epididymal spermatozoa a greater proportion of female embryos was observed, which was similar to the reference embryos. The shift observed on sex ratio toward female for those two groups was also
observed when they were compared with the expected 1:1 ratio (P < 0.05). The results showed the possibility to produced in vitro embryos using cryopreseved spermatozoa from epididymides and stored for long period of time at 5 .C. These procedures became an
important tool for animal preservation when the sperm cells cannot be cryopreserved immediately after the animal' s death. MenosThe objetive of the present study was to evaluate the effect of the interval between animal' s death and sperm recovery on the freezability and fertilizing ability of spermatozoa frombull epididymides stored for different periods of time. Testis from 25 bulls were collected at the abattoir 2 h after the slaughter. In the laboratory spermatozoa from one epididymis were recovered and analysed for motility. The remaining epididymis was stored for 24 h (G24), 48 h (G48) and 72 h (G72) at 5 .C. At the end of each time period, spermatozoa were recuperated and cryopreserved in Tris-egg yolk and glycerol. Pre-freeze and post-thaw sperm samples were taken to assess total and progressive motility, concentration, membrane integrity and acrosome integrity. For evaluation of fertilizing ability, in each time period five straws of each bullwere thawed, pooled
and used for in vitro embryo production. The results showed that after 48 h of storage there was a decline in total motility, which did not change until 72 h. Progressive motility, plasma membrane and acrosome integrity were not affected by any of the storage periods.
Conversely, all sperm parameters, except progressive motility, were reduced after cryopreservation. Embryo production was less (P < 0.05) in the treatments than in the reference group. However, there was no differences (P > 0.05) in blastoycst rate among
experimental groups. Considering all the embryos produced by epididymal spermatozoa a greater proportion of female em... Mostrar Tudo |
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Palavras-Chave: |
Agropecuária; Bovinae; Bull-dead; Espécie ameaçada; Fertilização In vitro; Sperm cryopreservation. |
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Thesagro: |
Bovino; Criopreservação; Esperma. |
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Thesaurus Nal: |
endangered species; in vitro fertilization; livestock. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03013naa a2200337 a 4500
001 1572315
005 2010-02-24
008 2009 bl --- 0-- u #d
100 1 $aMARTINS, C. F.
245 $aRecovery, cryopreservation and fertilization potential of bovine spermatozoa obtained from epididymides stored at 5 .C by different periods of time
260 $c2009
520 $aThe objetive of the present study was to evaluate the effect of the interval between animal' s death and sperm recovery on the freezability and fertilizing ability of spermatozoa frombull epididymides stored for different periods of time. Testis from 25 bulls were collected at the abattoir 2 h after the slaughter. In the laboratory spermatozoa from one epididymis were recovered and analysed for motility. The remaining epididymis was stored for 24 h (G24), 48 h (G48) and 72 h (G72) at 5 .C. At the end of each time period, spermatozoa were recuperated and cryopreserved in Tris-egg yolk and glycerol. Pre-freeze and post-thaw sperm samples were taken to assess total and progressive motility, concentration, membrane integrity and acrosome integrity. For evaluation of fertilizing ability, in each time period five straws of each bullwere thawed, pooled and used for in vitro embryo production. The results showed that after 48 h of storage there was a decline in total motility, which did not change until 72 h. Progressive motility, plasma membrane and acrosome integrity were not affected by any of the storage periods. Conversely, all sperm parameters, except progressive motility, were reduced after cryopreservation. Embryo production was less (P < 0.05) in the treatments than in the reference group. However, there was no differences (P > 0.05) in blastoycst rate among experimental groups. Considering all the embryos produced by epididymal spermatozoa a greater proportion of female embryos was observed, which was similar to the reference embryos. The shift observed on sex ratio toward female for those two groups was also observed when they were compared with the expected 1:1 ratio (P < 0.05). The results showed the possibility to produced in vitro embryos using cryopreseved spermatozoa from epididymides and stored for long period of time at 5 .C. These procedures became an important tool for animal preservation when the sperm cells cannot be cryopreserved immediately after the animal' s death.
650 $aendangered species
650 $ain vitro fertilization
650 $alivestock
650 $aBovino
650 $aCriopreservação
650 $aEsperma
653 $aAgropecuária
653 $aBovinae
653 $aBull-dead
653 $aEspécie ameaçada
653 $aFertilização In vitro
653 $aSperm cryopreservation
700 1 $aDRIESSEN, K.
700 1 $aCOSTA, P. M.
700 1 $aCARVALHO-NETO, J. O.
700 1 $aSOUSA, R. V.
700 1 $aRUMPF, R.
700 1 $aDODE, M. N.
773 $tAnimal Reproduction Science$gv. 116, n. 1-2, nov. 2009, p. 50-57
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Registro original: |
Embrapa Cerrados (CPAC) |
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| Registros recuperados : 37 | |
| 1. |  | MONTE, D. C.; ALMEIDA, E. R. P. Alimentos nutracêuticos e funcionais. In: ENCONTRO DA SOCIEDADE BRASILEIRA DE MELHORAMENTO DE PLANTAS REGIONAL DF, 1., 2005, Brasília, DF. [Anais...]. Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 27. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 144).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | CITADIN, C. T.; ALMEIDA, E. R. P.; DERNER, R. B.; MARTINS, N. F.; MONTE, D. C. Construção de biblioteca de cDNA e clonagem do gene da enzima Ä6-desaturase de ácidos graxos poliinsaturados ômega-3 da microalga marinha Thalassiosira fluviatilis. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 72.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | DERNER, R. B.; CITADIN, C.; ALMEIDA, E. R. P.; MONTE, D. C.; FETT, R. Determinação do perfil de ácidos graxos poliinsaturados das microalgas Chaetoceros Muelleri E Thalassiosira Fluviatilis e confecção de uma biblioteca de cDNA para cada alga. In: ENCONTRO DA SOCIEDADE BRASILEIRA DE MELHORAMENTO DE PLANTAS REGIONAL DF, 1., 2005, Brasília, DF. [Anais...]. Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 132. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 144).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | SILVA, M. L. da; YUYAMA, K.; ALMEIDA, E. R. P. de; ASTOLFI-FILHO, S. Mecanismos de resistência ativos em frutos de camu-camu (Myrciaria dúbia (H.B.K.) Mc Vaugh). In: CONGRESSO NACIONAL DE BOTÂNICA, 57.; ENCONTRO ESTADUAL DE BOTÂNICOS, 13.; ENCONTRO ESTADUAL DE HERBÁRIOS, 5., 2006, Gramado, RS. [Anais...]. Porto Alegre, RS: Sociedade Botânica do Brasil, 2006. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | GUERCHE, P.; ALMEIDA, E. R. P. de; SCHWARZTEIN, M. A.; GANDER, E. S.; KREBBERS, E.; PELLETIER, G. Expression of the 2s albumin from Bertholletia excelsa in Brassica napus. Molecular & General Genetics, Berlin, v.221, n.3, p.306-314, 1990. p.306-314| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | HOFFMANN, L. V.; GANDER, E. S.; ALMEIDA, E. R. P. de; SILVA FILHO, M. C.; VIEIRA, M. L. C. Fatores influenciando a regeneracao de plantas e expressao transiente de GUS na tranformacao de Stylosanthes guianensis por Agrobacterium tumefaciens. Brazilian Journal of Genetics, Ribeirao Preto, v.19, n.3, p.375, 1996. Supplement.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | ANGELO, P. C. da S.; ALMEIDA, E. R. P.; NUNES-SILVA, C. G.; ASSUNÇÃO, E. N.; ASTOLFI-FILHO, S. Primeira biblioteca de cDNA de frutos: etapa inicial para a análise do genoma funcional de guaranazeiro (Paullinia cupana var. sorbilis). In: ENCONTRO DE GENÉTICA DO AMAZONAS, 4.; ENCONTRO DE GENÉTICA DA REGIÃO NORTE, 1., 2003, Manaus. Livro de resumos. Manaus: SBG, 2003. p. 125.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 14. | | SOUZA, C. R.; ALMEIDA, E. R. de; SAMPAIO, M. J.; PEREIRA, P. A. A.; SA, M. F. G. de. Purificação, clonagem e expressão da arcelina em espécies de feijão susceptíveis ao ataque de carunchos: estudo comparativo da expressão obtida através de técnicas de retrocruzamento e engenharia genética. In: REUNIÃO BRASILEIRA SOBRE CONTROLE BIOLÓGICO DE DOENÇAS DE PLANTAS, 3., 1989, Piracicaba. Anais. Piracicaba: ESALQ, 1989. p. 97-98.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 15. | | DUSI, D. M. A.; DUBALD, M.; ALMEIDA, E. R. P. de; CALDAS, L. S.; GANDER, E. S. Transgenic plants of ramie (Boehmeria nivea Gand.) obtained by Agrobacterium mediated transformation. Plant Cell Reports, Berlin, v.12, n.l1, p.625-628, 1993. p.625-628| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | VINCENTZ, M.; LEITE, A.; MATTAR, S. C. M. da; NESHICH, G.; BARROS, L.; WEINBERG, D.; ALMEIDA, E. R. de; PAES, C. M. de; ARAGAO, F.; GANDER, E. S. ACGT and vicilin core sequences in an essential seed specific promoter region from Brazil nut albumin gene recognized by the opaque-2 regulatory protein. In: ENCUENTRO LATINO AMERICANO DE BIOTECNOLOGIOA VEGETAL, 2.,1995, Puerto Iguazu, Argentina. REDBIOS95. [S.1.:s.n.],1995. n.D-23.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | VINCENTZ, M.; LEITE, A.; NESHICH, G.; VRIED, G.; MATTAR, C.; BARROS, L.; WEINBERG, D.; ALMEIDA, E. R. de; PAES de CARVALHO, M.; ARAGÃO, F.; GANDER, E. ACGT and vicilin core sequences in a promoter domain required for seed-specific expression of a 2S storage protein gene are recognized by the opaque-2 regulatory protein. Plant Molecular Biology, v. 34, n. 6, p. 879-889, 1997.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | BARROS, L. M. G.; VINCENTZ, M.; WEINBERG, D. F.; CARVALHO, M. P. de; ALMEIDA, E. R. de; SA, M. F. G. de; GANDER, E. S. Analysis of a 2S seed storage protein gene promoter from Brazil nut. In: ENCONTRO BRASILEIRO DE BIOTECNOLOGIA VEGETAL, 1., 1993, Brasilia. Programa e resumos. Brasilia: EMBRAPA-CENARGEN, 1993. Poster 001. Resumo.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | MOURA, V. F. de; ALMEIDA, E. R. P.; YUYAMA, K.; VALADÃO, F.; ZAMUDIO, B. L.; MONTE, D. C.; SILVIA, M. L. cDNA library construction of Buriti fruit preliminaries analysis of ESTs (Expressed Sequence Tags). In: ENCUENTRO LATINO AMERICANO Y DEL CARIBE DE BIOTECNOLOGIA AGROPECUARIA: REDBIO 2007, 6., 2007, Viña del Mar, Chile. Abstracts and posters? Roma: FAO, 2007. Poster 615.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 37 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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